A nondamaging blood sampling technique for waterfowl embryos

نویسندگان

  • Nicolas Lecomte
  • Gilles Gauthier
  • Louis Bernatchez
  • Jean-François Giroux
چکیده

Development of minimally invasive techniques to collect blood in free-living birds is desirable for both ethical and conservation reasons. We describe a new, simple technique for collecting blood samples from waterfowl eggs that involves recovering blood from a small hemorrhage after puncturing the shell of eggs showing the first signs of hatching. This technique allowed us to obtain a large number of blood samples from hatchling Greater Snow Geese (Chen caerulescens atlantica) and did not require the sacrifice or retention of any individuals, thus minimizing stress. High-quality blood samples for genetic studies were obtained, with no adverse effects on either embryos or the post-hatch survival of young geese. SINOPSIS. Una nueva técnica para obtener muestras de sangre de embriones de gansos Es ético y deseable, en aras de conservar, el desarrollar técnicas poco invasivas para tomar muestras de sangre de aves vivas. Describimos una técnica para tomar muestras de sangre de huevos de gansos que consiste en tomar sangre de la pequeña hemorragia producida por una punción del cascaron cuando se observan lo primeros signos de eclosionar. Este técnica permitió tomar una muestra considerable de sangre de huevos de ganso (Chen caerulescens atlantica) sin requerir sacrificar o retener a ningún individuo, minimizando de esta forma el estrés causando por lo último. Las muestras tomadas fueron de alta calidad para análisis genético, sin que se observara efecto adverso alguno en los embriones o la supervivencia de los gansitos producto de los huevos manipulados y que eclosionaron.

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تاریخ انتشار 2006